Exposure controls include equipment and procedures designed to minimize inhalation, skin absorption, or ingestion of hazardous materials. Fume hoods and biosafety cabinets are two common pieces of lab equipment that can greatly decrease the potential for inhalation exposures to hazardous chemicals or biological agents. Although they may look similar, laboratory fume hoods and biosafety cabinets are designed and function very differently and must be used appropriately to afford the user protection. Many additional hazard-specific or process-related controls are available. Consult with EH&S (9-2553) to discuss control options. Show
Laboratory Fume Hoods Biosafety Cabinets Laboratory Fume HoodsA properly operating and correctly used fume hood can reduce or eliminate exposure to volatile liquids, dusts, and mists. It is advisable to use a laboratory hood when working with all hazardous substances. In addition, a laboratory hood or other suitable containment device must be used for all work with "particularly hazardous substances." Each fume hood should have a current calibration sticker and a marker indicating the highest sash height to be used when working with hazardous materials. Contact EH&S for a hood evaluation if these labels are missing. Each fume hood must be equipped with at least one type of continuous quantitative monitoring device designed to provide the user with current information on the operational status of the hood. These devices are typically a Magnehelic pressure gauge or a TSI digital flow meter. The fume hood is operating correctly when the Magnehelic gauge indicates the pressure level at the time of certification or when the TSI gauge reads 100 fpm or greater. The following work practices are always required when using chemical fume hoods; more stringent work practices may be necessary in some circumstances. Safe Fume Hood Work Practices
ResourcesFume Hood Fact Sheet - for labs in PSBFume Hood Fact Sheet - for labs in CBBFume Hood Fact Sheet - for all other labsTitle 8, California Code of Regulations, Section 5154.1 (8 CCR 5154.1), "Ventilation Requirements for Laboratory-Type Hood Operations" back to top Biosafety CabinetsBiosafety Cabinet Testing and CertificationBiological Safety Cabinets (BSCs) are tested and certified in accordance with NSF/ANSI Standard 49, "Biosafety Cabinetry Certification." Testing and certification must occur annually or any time a BSC is moved. For more information on BSC testing and certification, contact EH&S at 459-2553 or . The biosafety cabinet (BSC) is an essential piece of safety equipment for many laboratories conducting biological research. The BSC is designed to provide both personnel and environmental protection from potentially infectious materials and human pathogens. BSC's are manufactured in three different classes (Class I, II and III). The common feature in all BSCs is the high efficiency particulate air (HEPA) filter. HEPA filters can remove particles down to 0.3 microns with 99.97% efficiency and will trap most bacteria and viruses. Vapors (from ethanol, formalin, etc) and gases will not be captured and removed by the HEPA filter. Class I Biosafety CabinetProvide personnel and environmental protection, but do not provide a sterile work surface. Airflow in a Class I BSC is similar to a chemical fume hood, with air being drawn away from the worker and across the work surface. The exhaust from the cabinet is HEPA filtered and this protects the environment. Class I BSC are useful for work that requires containment but not product sterility. Class II Biosafety CabinetProvide personnel, product and environmental protection. Air is drawn around the worker into the front grill of the BSC, providing operator protection. HEPA filtered sterile air flows down onto the work surface, minimizing the potential for cross-contamination. Exhaust air passes through another HEPA filter before being released into the surrounding environment. Class II units are further divided into four types; A1, A2, B1, and B2, based on the percentage of air that is recirculated and exhausted. Class III Biosafety CabinetClass III BSCs are designed to provide maximum protection to the worker and the environment. Sometimes called Class III glove boxes, these units are gas-tight enclosures with a non-opening view window. Intake air is filtered through a HEPA filter and exhaust air passes through two HEPA filters before being released. Safe Biosafety Cabinet Work Practices
Common BSC Work Practice Errors
ResourcesTitle 8, California Code of Regulations, Section 5154.2 (8 CCR 5154.2), "Ventilation Requirements for Biological Safety Cabinets" Biosafety in Microbiological and Biomedical Laboratories (BMBL) 5th Edition Appendix A - Primary Containment for Biohazards: Selection, Installation and Use of Biological Safety Cabinets Workinig safely with Biosafety Cabinet videos: UC Davis Cornell back to top How frequently should your biosafety cabinet be certified?BSCs must be certified when installed, whenever they are moved and at least annually [29 CFR 1910.1030(e)(2) (iii)(B)]. Employers should ensure that a risk assessment has been completed and approved for the work to be conducted and to identify the class and type of BSC needed for the operation or procedure.
Which of the following is a common feature of all biosafety cabinets quizlet?The common feature in all BSCs is the high efficiency particulate air (HEPA) filter. HEPA filters can remove particles down to 0.3 microns with 99.97% efficiency and will trap most bacteria and viruses.
What is the purpose of a biosafety cabinet in a microbiology laboratory?Biosafety cabinets (BSCs) are one type of biocontainment equipment used in biological laboratories to provide personnel, environmental, and product protection.
What are the standardized classifications of biosafety cabinets?Biosafety cabinets are divided into three classes: I, II and III. Class I provides protection for the user and surrounding environment, but no protection for the sample being manipulated. Class II provides protection for the user, environment and sample, and is divided into four types: A1, A2, B1 and B2.
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